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Product Name
MID1 Polyclonal Antibody
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Description
Polyclonal antibody to MID1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MID1 antibody; BBBG1 antibody; FXY antibody; GBBB1 antibody; MIDIN antibody; OGS1 antibody; OS antibody; OSX antibody; RNF59 antibody; TRIM18 antibody; XPRF antibody; ZNFXY antibody; midline 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - MID1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MID1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunofluorescence - MID1 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using MID1 antibody . Blue: DAPI for nuclear staining.
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Background
Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"