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Product Name
MICAL2 antibody
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Description
MICAL2 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IF detected in HepG2 cells. Positive IP detected in PC-3 cells. Positive WB detected in PC-3 cells. Observed molecular weight by Western-blot: 95, 112 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686H03148 antibody; DKFZp686H2469 antibody; KIAA0750 antibody; MICAL2 antibody; MICAL2PV1 antibody; MICAL2PV2 antibody; Protein MICAL 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MICAL2 recombinant protein (Accession Number: XM_017018589). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human brain using Catalog No:112610(MICAL2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:112610(MICAL2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MICAL2 antibody Catalog No:112610 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112610(MICAL2 Antibody) at dilution of 1:300
IP Result of anti-MICAL2 (IP:Catalog No:112610, 5ug; Detection:Catalog No:112610 1:300) with PC-3 cells lysate 5000ug.
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Background
MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain and a Lin11, Isl-1 and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variants of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"