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Product Name
MIB2 antibody
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Description
MIB2 Rabbit Polyclonal antibody. Positive WB detected in mouse skeletal muscle tissue. Positive IP detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ20648 antibody; FLJ39787 antibody; MIB2 antibody; Mind bomb homolog 2 antibody; Novel zinc finger protein antibody; Novelzin antibody; SKD antibody; Skeletrophin antibody; ZZANK1 antibody; ZZZ5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MIB2 recombinant protein (Accession Number: XM_047446743). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:112607(MIB2 antibody) at dilution of 1:500
IP Result of anti-MIB2 (IP:Catalog No:112607, 4ug; Detection:Catalog No:112607 1:500) with mouse skeletal muscle tissue lysate 2200ug.
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Background
MIB2, also named as E3 ubiquitin-protein ligase MIB2, is a 1013 amino aicd protein, which Contains 9 ANK repeats and 2 MIB/HERC2 domains. MIB2 as E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins.MIB2 positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB2 is expressed in skeletal muscle, and to a lesser extent in heart, brain and kidney. MIB2 exists as some isoforms and isoform 9 is about 73 kDa.
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