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Product Name
mH2A2 Polyclonal Antibody
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Description
Polyclonal antibody to mH2A2
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Tested applications
WB, FC
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Species reactivity
Human, Mouse
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Alternative names
H2AFY2 antibody; macroH2A2 antibody; core histone macro-H2A.2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human H2AFY2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
FC 1:20 - 1:50 -
Validations
Western blot - mH2A2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using H2AFY2 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"