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Product Name
MGAT5B antibody
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Description
MGAT5B Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in Jurkat cells. Positive WB detected in HepG2 cells, C6 cells, Jurkat cells, MCF7 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 89-100 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
FLJ25132 antibody; GlcNAc T Vb antibody; GnT IX antibody; GnT VB antibody; hGnTVb antibody; KIAA2008 antibody; MGAT5B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MGAT5B recombinant protein (Accession Number: BC062354). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112596(MGAT5B antibody) at dilution of 1:500
IP Result of anti-MGAT5B (IP:Catalog No:112596, 4ug; Detection:Catalog No:112596 1:700) with Jurkat cells lysate 3600ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:112596(MGAT5B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MGAT5B represents mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B
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References
- Kippe JM, Mueller TM, Haroutunian V, Meador-Woodruff JH. Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia. Schizophrenia research. 166(1-3):219-24. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"