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Product Name
MGAT2 Polyclonal Antibody
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Description
Polyclonal antibody to MGAT2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
MGAT2 antibody; CDG2A antibody; CDGS2 antibody; GLCNACTII antibody; GNT-II antibody; GNT2 antibody; alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 178-447 of human MGAT2 (NP_002399.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - MGAT2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MGAT2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Plays an essential role in protein N-glycosylation. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety in the core structure of the nascent N-linked glycan chain, giving rise to the second branch in complex glycans.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"