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Product Name
MGAT1 Polyclonal Antibody
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Description
Polyclonal antibody to MGAT1
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Tested applications
WB
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Species reactivity
Human
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Alternative names
MGAT1 antibody; GLCNAC-TI antibody; GLCT1 antibody; GLYT1 antibody; GNT-1 antibody; GNT-I antibody; GnTI antibody; MGAT antibody; alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - MGAT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MGAT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"