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Product Name
MGAT1 antibody
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Description
MGAT1 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, HepG2 cells. Positive IHC detected in human liver cancer tissue, human heart tissue, mouse heart tissue, mouse liver tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 51-55 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GGNT1 antibody; GlcNAc T I antibody; GLCNAC TI antibody; GLCT1 antibody; GLYT1 antibody; GNT 1 antibody; GNT I antibody; MGAT antibody; MGAT1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MGAT1 recombinant protein (Accession Number: XM_005265916). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:112535(MGAT1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:112535(MGAT1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:112535(MGAT1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using MGAT1 antibody Catalog No:112535 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
MGAT1 represents mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
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References
- Urita A, Matsuhashi T, Onodera T. Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage. Arthritis and rheumatism. 63(11):3428-38. 2011.
- Kippe JM, Mueller TM, Haroutunian V, Meador-Woodruff JH. Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia. Schizophrenia research. 166(1-3):219-24. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"