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Product Name
METTL20 Polyclonal Antibody
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Description
Polyclonal antibody to METTL20
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Tested applications
WB, IHC
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Species reactivity
Mouse, Rat
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Alternative names
ETFBKMT antibody; C12orf72 antibody; ETFB-KMT antibody; METTL20 antibody; electron transfer flavoprotein beta subunit lysine methyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 105-262 of human METTL20 (NP_001129335.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - METTL20 Polyclonal Antibody
Western blot analysis of extracts of mouse heart, using METTL20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - METTL20 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using METTL20 antibody at dilution of 1:100 (40x lens).
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Background
Protein-lysine methyltransferase that selectively trimethylates the flavoprotein ETFB in mitochondria. Thereby, may negatively regulate the function of ETFB in electron transfer from Acyl-CoA dehydrogenases to the main respiratory chain.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"