Human MAP1D (His Tag) recombinant protein

Human MAP1D (His Tag) recombinant protein

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Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.

Methionine aminopeptidase 1D, mitochondrial

CDS OF METAP-3 WITHIN PCR FRAGMENT; MAP1D

Q6UB28

METAP1D; MAP1D

Baculovirus-Insect Cells

A DNA sequence encoding the human MAP1D (NP_954697.1) (Arg 44-Ala 335) was expressed, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human MAP1D consists of 303 amino acids and predicts a molecular mass of 33.4 kDa. It migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC, R&D Systems, Catalog#ES017. The resulting GP-AMC is cleaved by human DPPIV/CD26, R&D Systems, Catalog#1180SE. The specific activity is >30 pmoles/min/μg.