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Product Name
Melanophilin antibody
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Description
Melanophilin Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue. Positive IF detected in HeLa cells. Positive IP detected in A375 cells. Positive WB detected in A375 cells. Observed molecular weight by Western-blot: 66 kDa, 80 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Exophilin 3 antibody; melanophilin antibody; MLPH antibody; SLAC2 A antibody; SLAC2A antibody; Synaptotagmin like protein 2a antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Melanophilin recombinant protein (Accession Number: NM_024101). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:112651(MLPH antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:112651(MLPH antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:112651(MLPH antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:112651(MLPH Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-MLPH (IP:Catalog No:112651, 4ug; Detection:Catalog No:112651 1:600) with A375 cells lysate 3600ug.
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Background
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.
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References
- Westbroek W, Tuchman M, Tinloy B. A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome. Molecular genetics and metabolism. 94(2):248-54. 2008.
- Van Gele M, Geusens B, Schmitt AM, Aguilar L, Lambert J. Knockdown of myosin Va isoforms by RNAi as a tool to block melanosome transport in primary human melanocytes. The Journal of investigative dermatology. 128(10):2474-84. 2008.
- Wang J, Qin R, Ma Y. Differential gene expression in normal esophagus and Barrett's esophagus. Journal of gastroenterology. 44(9):897-911. 2009.
- Westbroek W, Klar A, Cullinane AR. Cellular and clinical report of new Griscelli syndrome type III cases. Pigment cell & melanoma research. 25(1):47-56. 2012.
- Cetica V, Hackmann Y, Grieve S. Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding. The Journal of allergy and clinical immunology. 135(5):1310-8.e1. 2015.
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