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Product Name
MEK5 antibody
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Description
MEK5 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human testis tissue. Positive IF detected in Hela cells. Positive WB detected in HeLa cells, A431 cells, HepG2 cells. Observed molecular weight by Western-blot: 55kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
HsT17454 antibody; MAP kinase kinase 5 antibody; MAP2K5 antibody; MAPK/ERK kinase 5 antibody; MAPKK 5 antibody; MAPKK5 antibody; MEK 5 antibody; MEK5 antibody; MKK5 antibody; PRKMK5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MEK5 recombinant protein (Accession Number: NM_145160). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112647(MAP2K5 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human heart using Catalog No:112647(MAP2K5 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:112647(MAP2K5 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using MAP2K5 antibody Catalog No:112647 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
MAP2K5 represents mitogen-activated protein kinase kinase 5
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"