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Product Name
MED30 antibody
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Description
MED30 Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, Transfected HEK-293 cells. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 20-25 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
MED30 antibody; mediator complex subunit 30 antibody; THRAP6 antibody; TRAP25 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MED30 recombinant protein (Accession Number: NM_080651). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112590(MED30 antibody) at dilution of 1:200
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112590(MED30 Antibody) at dilution of 1:1000
IP Result of anti-MED30 (IP:Catalog No:112590, 4ug; Detection:Catalog No:112590 1:500) with mouse heart tissue lysate 4000ug.
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Background
MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa..
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References
- Lee YJ, Han ME, Baek SJ, Kim SY, Oh SO. MED30 Regulates the Proliferation and Motility of Gastric Cancer Cells. PloS one. 10(6):e0130826. 2015.
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