MED22 Polyclonal Antibody

Cat.#: 161811

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Product Information

  • Product Name
    MED22 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to MED22
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse
  • Alternative names
    MED22 antibody; MED24 antibody; SURF5 antibody; surf-5 antibody; SRB6 antibody; mediator complex subunit 22 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human MED22 (NP_852468.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - MED22 Polyclonal Antibody

    Western blot - MED22 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using MED22 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

  • Background
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"