Cart 2

MECP2 antibody

Cat.#: 112577

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    MECP2 antibody
  • Documents
  • Description
    MECP2 Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in MDA-MB-453s cells, human brain tissue, mouse heart tissue, mouse lung tissue, mouse ovary tissue, SH-SY5Y cells. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human brain tissue. Positive FC detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 75 kDa
  • Tested applications
    ELISA, IHC, WB, IF, IP, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AUTSX3 antibody; DKFZp686A24160 antibody; MeCp 2 protein antibody; MECP2 antibody; Methyl CpG binding protein 2 antibody; MRX16 antibody; MRX79 antibody; MRXS13 antibody; MRXSL antibody; PPMX antibody; RTS antibody; RTT antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MECP2 recombinant protein (Accession Number: NM_004992). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112577(MECP2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112577(MECP2 antibody) at dilution of 1:50 (under 10x lens)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:112577(MECP2 Antibody) at dilution of 1:600

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with Catalog No:112577(MECP2 Antibody) at dilution of 1:600

    IP Result of anti-MECP2 (IP:Catalog No:112577, 4ug; Detection:Catalog No:112577 1:500) with MCF-7 cells lysate 1280ug.

    IP Result of anti-MECP2 (IP:Catalog No:112577, 4ug; Detection:Catalog No:112577 1:500) with MCF-7 cells lysate 1280ug.

    Immunofluorescent analysis of HepG2 cells using Catalog No:112577(MECP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:112577(MECP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 NIH/3T3 cells were stained with 0.2ug MECP2 antibody (Catalog No:112577, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 NIH/3T3 cells were stained with 0.2ug MECP2 antibody (Catalog No:112577, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins.
  • References
    • Wang Y, Liu C, Guo QL. Intrathecal 5-azacytidine inhibits global DNA methylation and methyl- CpG-binding protein 2 expression and alleviates neuropathic pain in rats following chronic constriction injury. Brain research. 1418:64-9. 2011.
    • Leoh LS, van Heertum B, De Rijck J. The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity. Molecular cancer research : MCR. 10(3):378-91. 2012.
    • Basu A, Woods-Burnham L, Ortiz G. Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2. Clinical immunology (Orlando, Fla.). 161(2):241-50. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"