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Product Name
MDMX antibody
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Description
MDMX Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, Apoptosised HeLa cells, HepG2 cells, Raji cells. Observed molecular weight by Western-blot: 80kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp781B1423 antibody; Double minute 4 protein antibody; HDMX antibody; Mdm2 like p53 binding protein antibody; MDM4 antibody; MDMX antibody; MRP1 antibody; p53 binding protein Mdm4 antibody; Protein Mdm4 antibody; Protein Mdmx antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MDMX recombinant protein (Accession Number: XM_024447115). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112573(MDM4 antibody) at dilution of 1:500
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Background
MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.
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References
- Ling X, Xu C, Fan C, Zhong K, Li F, Wang X. FL118 induces p53-dependent senescence in colorectal cancer cells by promoting degradation of MdmX. Cancer research. 74(24):7487-97. 2014.
- Wu W, Xu C, Ling X. Targeting RING domains of Mdm2-MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells. Cell death & disease. 6:e2035. 2015.
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