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MDM2 antibody

Cat.#: 112572

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Product Information

  • Product Name
    MDM2 antibody
  • Documents
  • Description
    MDM2 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, A549 cells, MCF-7 cells. Observed molecular weight by Western-blot: 30-55 kDa
  • Tested applications
    ELISA, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Double minute 2 protein antibody; hdm2 antibody; HDMX antibody; MDM2 antibody; Oncoprotein Mdm2 antibody; p53 binding protein Mdm2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_002392). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

  • Validations

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:112572(MDM2 Antibody) at dilution of 1:300

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:112572(MDM2 Antibody) at dilution of 1:300

  • Background
    MDM2 belongs to the MDM2/MDM4 family. It inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. The mdm2 oncogene encodes a 90 kDa protein that can bind to the p53 tumor suppressor protein and negatively regulate its functions in transcription, cell cycle arrest, and apoptosis. The human mdm2 can be cleaved into a 68 kDa fragment and a 32 kDa fragment.(PMID:9278461).The antibody recognize all the isoforms of MDM2 except isoform Mdm2-E.
  • References
    • Zhang J, Sun Q, Zhang Z, Ge S, Han ZG, Chen WT. Loss of microRNA-143/145 disturbs cellular growth and apoptosis of human epithelial cancers by impairing the MDM2-p53 feedback loop. Oncogene. 32(1):61-9. 2013.
    • Zhang XF, Pan QZ, Pan K. Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma. Molecular carcinogenesis. 55(1):64-76. 2016.
    • Jin Y, Cao B, Zhang M. RASSF10 suppresses hepatocellular carcinoma growth by activating P53 signaling and methylation of RASSF10 is a docetaxel resistant marker. Genes & cancer. 6(5-6):231-40. 2015.
    • Wang Y, Chen CL, Pan QZ. Decreased TPD52 expression is associated with poor prognosis in primary hepatocellular carcinoma. Oncotarget. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"