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Product Name
MDC1 Polyclonal Antibody
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Description
Polyclonal antibody to MDC1
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
MDC1 antibody; NFBD1 antibody; mediator of DNA damage checkpoint 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - MDC1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MDC1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 5s.
Immunohistochemistry - MDC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MDC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MDC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MDC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MDC1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - MDC1 Polyclonal Antibody
Immunofluorescence analysis of U-2 OS cells using MDC1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.
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