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Product Name
MDC1 antibody
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Description
MDC1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human appendix tissue, human breast cancer tissue. Positive IF detected in HEK-293 cells, HeLa cells.
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Tested applications
ELISA, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp781A0122 antibody; KIAA0170 antibody; MDC1 antibody; NFBD1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MDC1 recombinant protein (Accession Number: BC152556). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:112567(MDC1 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:112567(MDC1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:112567(MDC1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.
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