Cart 2

MCM10 antibody

Cat.#: 112551

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    MCM10 antibody
  • Documents
  • Description
    MCM10 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 97-105 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CNA43 antibody; DNA43 antibody; HsMCM10 antibody; MCM10 antibody; PRO2249 antibody; Protein MCM10 homolog antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MCM10 recombinant protein (Accession Number: BC009108). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IF: 1:50-1:500

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112551(MCM10 antibody) at dilution of 1:300

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112551(MCM10 antibody) at dilution of 1:300

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112551(MCM10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:112551(MCM10 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    MCM10, also named as HsMCM10, belongs to the MCM10 family. It acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, it plays a role in preventing DNA damage during replication and is essential for the integrity of the RECQ4–MCM replicative helicase complex. It mediates RECQ4 association with MCM2-7 helicase complex during DNA replication.
  • References
    • Xu X, Rochette PJ, Feyissa EA, Su TV, Liu Y. MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication. The EMBO journal. 28(19):3005-14. 2009.
    • Mazurek A, Luo W, Krasnitz A, Hicks J, Powers RS, Stillman B. DDX5 regulates DNA replication and is required for cell proliferation in a subset of breast cancer cells. Cancer discovery. 2(9):812-25. 2012.
    • Anantha RW, Alcivar AL, Ma J. Requirement of heterogeneous nuclear ribonucleoprotein C for BRCA gene expression and homologous recombination. PloS one. 8(4):e61368. 2013.
    • Wang JT, Xu X, Alontaga AY, Chen Y, Liu Y. Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction. Cell reports. 7(3):848-58. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"