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Product Name
MCCC2 Polyclonal Antibody
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Description
Polyclonal antibody to MCCC2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
MCCC2 antibody; MCCB antibody; methylcrotonoyl-CoA carboxylase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 284-563 of human MCCC2 (NP_071415.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - MCCC2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MCCC2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Western blot - MCCC2 Polyclonal Antibody
Western blot analysis of extracts of various cells, using MCCC2 antibody at 1:1000 dilution ratio through one-step method.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - MCCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using MCCC2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MCCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using MCCC2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MCCC2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using MCCC2 antibody at dilution of 1:100 (40x lens).
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Background
Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"