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Product Name
MCC antibody
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Description
MCC Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in PC-3 cells. Positive IP detected in PC-3 cells. Positive WB detected in human brain tissue, COLO 320 cells, mouse liver tissue, PC-3 cells. Observed molecular weight by Western-blot: 98 kDa; 113-118 kDa
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Tested applications
ELISA, IHC, IP, WB, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DKFZp762O1615 antibody; FLJ38893 antibody; FLJ46755 antibody; MCC antibody; MCC1 antibody; mutated in colorectal cancers antibody; Protein MCC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MCC recombinant protein (Accession Number: NM_002387). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112542(MCC antibody) at dilution of 1:500
IP Result of anti-MCC (IP:Catalog No:112542, 3ug; Detection:Catalog No:112542 1:500) with PC-3 cells lysate 5000ug.
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112542(MCC antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:112542(MCC Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,
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References
- Rohrbeck A, Borlak J. Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1. PloS one. 4(10):e7315. 2009.
- Corbo C, Orrù S, Gemei M. Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity. Proteomics. 12(12):2045-59. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"