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Product Name
MBD2 antibody
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Description
MBD2 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, A431 cells, HEK-293 cells, human brain tissue, RAW264.7 cells. Observed molecular weight by Western-blot: 43-45 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
Demethylase antibody; DKFZp586O0821 antibody; DMTase antibody; MBD2 antibody; NY CO 41 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_003927). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112502(MBD2 antibody) at dilution of 1:1000
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Background
MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"