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Product Name
MAX antibody
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Description
MAX Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A549 cells, PC-3 cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bHLHd4 antibody; bHLHd5 antibody; bHLHd6 antibody; bHLHd7 antibody; bHLHd8 antibody; MAX antibody; MYC assOCiated factor X antibody; orf1 antibody; Protein max antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAX recombinant protein (Accession Number: NM_145112). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112500(MAX antibody) at dilution of 1:300
IP Result of anti-MAX (IP:Catalog No:112500, 4ug; Detection:Catalog No:112500 1:300) with HEK-293 cells lysate 1200ug.
Immunofluorescent analysis of PC-3 cells using Catalog No:112500(MAX Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"