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Product Name
MAVS; VISA antibody
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Description
MAVS; VISA Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, human heart tissue, Jurkat cells. Positive IF detected in Hela cells. Positive IHC detected in human skin tissue. Observed molecular weight by Western-blot: 70-75kd
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Tested applications
ELISA, WB, IF, IP, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CARDIF antibody; DKFZp547C224 antibody; DKFZp666M015 antibody; FLJ27482 antibody; FLJ35386 antibody; FLJ38051 antibody; FLJ41962 antibody; IPS 1 antibody; IPS1 antibody; KIAA1271 antibody; MAVS antibody; VISA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAVS; VISA recombinant protein (Accession Number: NM_020746). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112499(MAVS; VISA antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using MAVS antibody Catalog No:112499 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MAVS; VISA (IP:Catalog No:112499, 3ug; Detection:Catalog No:112499 1:1000) with HEK-293 cells lysate 1700ug.
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:112499(MAVS; VISA Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:112499(MAVS; VISA Antibody) at dilution of 1:200 (under 40x lens)
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Background
Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or interferon beta promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.
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References
- Dong J, Xu S, Wang J. Porcine reproductive and respiratory syndrome virus 3C protease cleaves the mitochondrial antiviral signalling complex to antagonize IFN-β expression. The Journal of general virology. 96(10):3049-58. 2015.
- Hwang KY, Choi YB. Modulation of Mitochondrial Antiviral Signaling by Human Herpesvirus 8 Interferon Regulatory Factor 1. Journal of virology. 90(1):506-20. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"