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Product Name
MAT2B antibody
- Documents
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Description
MAT2B Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, mouse testis tissue. Positive IP detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human lung tissue, human kidney tissue. Observed molecular weight by Western-blot: 38 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MAT II antibody; MAT II beta antibody; MAT2B antibody; MATIIbeta antibody; Nbla02999 antibody; SDR23E1 antibody; TGR antibody; UNQ2435/PRO4995 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAT2B recombinant protein (Accession Number: NM_182796). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112494(MAT2B antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lung using Catalog No:112494(MAT2B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:112494(MAT2B antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MAT2B antibody Catalog No:112494 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MAT2B (IP:Catalog No:112494, 4ug; Detection:Catalog No:112494 1:500) with HepG2 cells lysate 1000ug.
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Background
MAT2B represents methionine adenosyltransferase II, beta
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References
- Li X, Sun Q, Li X. Dietary betaine supplementation to gestational sows enhances hippocampal IGF2 expression in newborn piglets with modified DNA methylation of the differentially methylated regions. European journal of nutrition. 54(7):1201-10. 2015.
- Jia Y, Song H, Gao G, Cai D, Yang X, Zhao R. Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. Journal of agricultural and food chemistry. 63(46):10152-60. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"