Mastoparan M peptide

Mastoparan M peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

INLKAIAALAKKLL

H-Ile-Asn-Leu-Lys-Ala-Ile-Ala-Ala-Leu-Ala-Lys-Lys-Leu-Leu-OH

14

98%

C₇₀H₁₃₀N₁₈O₁₆

1478.9

Synthetic

Powder

Active against Gram-positive S. aureus ATCC25923, E. faecalis ATCC29212, S. aureus IS 10# (MIC 2.5-5 uM), S. aureus IS 37#, Gram-negative E. coli ATCC25922, E. cloacae ATCC13047, K. pneumonia ATCC700603 (MIC 10-20 uM), B. pyocyaneus CMCCB1010 (MIC 40 uM), P. aeruginosa ATCC27853, B. dysenteriae (MIC 5 uM), E. coli IS 37# (MIC 10 uM), E. coli IS 117# (MIC 40 uM), E. coli IS 218#, P. aeruginosa IS 350# (MIC 20 uM), K. pneumonia IS 59# (MIC 10 uM), fungi C. albicans ATCC2002, C. albicans ATCC90028 (MIC 10 uM), and C. parapsilosis ATCC22019 (MIC 40 uM) (Yang et al., 2013). Mastoparan M made in all D-amino acids showed 2-fold higher antibacterial activity than L-mastoparan M (Li ML et al 2000 Int J Antimicrob Agents. 13(3):203-8). The same peptide is also expressed in Vespa magnifica (Xu X et al, 2006) and Vespa tropica (Yang et al., 2013). Found in multiple species. Updated 3/2014; 4/2014 Mastoparan M has Antimicrobial activity. The source of Mastoparan M is Hornet Vespa mandarinia ; venom gland, the social wasp Vespa tropica; wasp venom, Vespa magnifica.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Xu X, Yang H, Yu H, Li J, Lai R. The mastoparanogen from wasp. Peptides. 2006;27(12):3053-7.
• Xu X, Yang H, Yu H, Li J, Lai R. The mastoparanogen from wasp. Peptides. 2006;27(12):3053-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.