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Product Name
MAST3 antibody
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Description
MAST3 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, human brain tissue, mouse lung tissue. Positive IP detected in Jurkat cells. Positive IHC detected in human kidney tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 144kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0561 antibody; MAST3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_015016). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112491(MAST3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112491(MAST3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:112491(MAST3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MAST3 antibody Catalog No:112491 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-MAST3 (IP:Catalog No:112491, 4ug; Detection:Catalog No:112491 1:500) with Jurkat cells lysate 4000ug.
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Background
MAST3, also named as KIAA0561, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. MAST3 catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"