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Mast Cell Chymase antibody

Cat.#: 112489

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Product Information

  • Product Name
    Mast Cell Chymase antibody
  • Documents
  • Description
    Mast Cell Chymase Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, human heart tissue, human placenta tissue, MCF7 cells. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 27-30 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Alpha chymase antibody; chymase antibody; chymase 1 antibody; mast cell antibody; CMA1 antibody; CYH antibody; CYM antibody; Mast Cell Chymase antibody; Mast cell protease I antibody; Mcpt 5 antibody; Mcpt5 antibody; MCT1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Mast Cell Chymase recombinant protein (Accession Number: NM_001836). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

  • Validations

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112489(CMA1 antibody) at dilution of 1:500

    mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112489(CMA1 antibody) at dilution of 1:500

    IP Result of anti-CMA1 (IP:Catalog No:112489, 4ug; Detection:Catalog No:112489 1:300) with mouse heart tissue lysate 2400ug.

    IP Result of anti-CMA1 (IP:Catalog No:112489, 4ug; Detection:Catalog No:112489 1:300) with mouse heart tissue lysate 2400ug.

  • Background
    Mast Cell Chymase, also named as CYH, CYM and CMA1, belongs to the peptidase S1 family and Granzyme subfamily. It is the major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. CMA1 maybe a target for cardiovascular disease therapies.
  • References
    • Baumann A, Gönnenwein S, Bischoff SC. The circadian clock is functional in eosinophils and mast cells. Immunology. 140(4):465-74. 2013.
    • Smith ET, Perry ET, Sears MB, Johnson DA. Expression of recombinant human mast cell chymase with Asn-linked glycans in glycoengineered Pichia pastoris. Protein expression and purification. 102:69-75. 2014.
    • Shi H, Jiang H, Wang L. Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device. Cell cycle (Georgetown, Tex.). 14(19):3058-65. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"