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Product Name
MARCKSL1 Polyclonal Antibody
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Description
Polyclonal antibody to MARCKSL1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MARCKSL1 antibody; F52 antibody; MACMARCKS antibody; MLP antibody; MLP1 antibody; MRP antibody; MARCKS like 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human MARCKSL1 (NP_075385.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - MARCKSL1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MARCKSL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
Immunofluorescence - MARCKSL1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MARCKSL1 antibody . Blue: DAPI for nuclear staining.
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Background
Controls cell movement by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. When unphosphorylated, induces cell migration (By similarity). When phosphorylated by MAPK8, induces actin bundles formation and stabilization, thereby reducing actin plasticity, hence restricting cell movement, including neuronal migration (By similarity). May be involved in coupling the protein kinase C and calmodulin signal transduction systems (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"