Product Name

Marcks antibody

Documents

Description

Marcks Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue, mouse pancreas tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 88kd

Tested applications

ELISA, WB, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

Macs antibody; Marcks antibody; PKCSL antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Recombinant Protein (Accession Number: NM_008538). Purification method: Protein A purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IHC: 1:20-1:200

Validations

mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112449(Marcks antibody) at dilution of 1:2000

Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112449(Marcks antibody) at dilution of 1:200 (under 10x lens)

Background

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS

References

Bai T, Luoh SW. GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation. Carcinogenesis. 29(3):473-9. 2008.
Gatlin JC, Estrada-Bernal A, Sanford SD, Pfenninger KH. Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding. Molecular biology of the cell. 17(12):5115-30. 2006.
Musse AA, Gao W, Rangaraj G, Boggs JM, Harauz G. Myelin basic protein co-distributes with other PI(4,5)P2-sequestering proteins in Triton X-100 detergent-resistant membrane microdomains. Neuroscience letters. 450(1):32-6. 2009.
Estrada-Bernal A, Gatlin JC, Sunpaweravong S, Pfenninger KH. Dynamic adhesions and MARCKS in melanoma cells. Journal of cell science. 122(Pt 13):2300-10. 2009.
Lejonklou MH, Barbu A, Stålberg P, Skogseid B. Accelerated proliferation and differential global gene expression in pancreatic islets of five-week-old heterozygous Men1 mice: Men1 is a haploinsufficient suppressor. Endocrinology. 153(6):2588-98. 2012.
Jin BY, Lin AJ, Golan DE, Michel T. MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability. Proceedings of the National Academy of Sciences of the United States of America. 109(37):14864-9. 2012.

Marcks antibody

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