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Product Name
MAP3K12 Polyclonal Antibody
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Description
Polyclonal antibody to MAP3K12
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Tested applications
WB
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Species reactivity
Human
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Alternative names
MAP3K12 antibody; DLK antibody; MEKK12 antibody; MUK antibody; ZPK antibody; ZPKP1 antibody; mitogen-activated protein kinase kinase kinase 12 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human MAP3K12 (NP_001180440.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - MAP3K12 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MAP3K12 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Part of a non-canonical MAPK signaling pathway. Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1. May be an activator of the JNK/SAPK pathway.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"