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Product Name
MAN2A1 Polyclonal Antibody
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Description
Polyclonal antibody to MAN2A1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
MAN2A1 antibody; AMan II antibody; GOLIM7 antibody; MANA2 antibody; MANII antibody; AManII antibody; alpha-mannosidase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 865-1144 of human MAN2A1 (NP_002363.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:1000 - 1:2000
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Validations
Western blot - MAN2A1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MAN2A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"