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Product Name
MAN1A2 antibody
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Description
MAN1A2 Rabbit Polyclonal antibody. Positive WB detected in human placenta tissue. Positive IHC detected in human placenta tissue, human heart tissue, human lung tissue, human ovary tissue. Observed molecular weight by Western-blot: 73kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
Alpha 1 antibody; 2 mannosidase IB antibody; MAN1A2 antibody; MAN1B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAN1A2 recombinant protein (Accession Number: NM_006699). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:112464(MAN1A2 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112464(MAN1A2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human placenta using Catalog No:112464(MAN1A2 antibody) at dilution of 1:50 (under 40x lens)
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Background
The first group or Class I mannosidase is comprised of α1–2 mannosidases from mammals and yeast with encoded polypeptides of 65–75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1–2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"