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Product Name
MALT1 antibody
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Description
MALT1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, mouse testis tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, IHC, IF, WB, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434L132 antibody; MALT1 antibody; MLT antibody; MLT1 antibody; Paracaspase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MALT1 recombinant protein (Accession Number: NM_173844). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112459(MALT1 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112459(MALT1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using MALT1 antibody Catalog No:112459 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HeLa cells were stained with 0.2ug MALT1 antibody (Catalog No:112459, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"