Rat MDHA / MDH1 (His Tag) recombinant protein

Rat MDHA / MDH1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Malate dehydrogenases 1(MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure. 

Malate dehydrogenase, cytoplasmic

MDHA; D17921; RP23-198N8.1; HEL-S-32; MGC:1375; MOR2; MOR-2; MDHL; B230377B03RIK; MDL1; MDH-S

O88989

Mdh1; Mdh

E. coli

A DNA sequence encoding the rat MDH1 (O88989) (Met 4-Ala 334) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat MDH1 comprises 345 amino acids and predicts a molecular mass of 38 kDa. The apparent molecular mass of the rat MDH1 is approximately 39 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE