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MAGED2 antibody

Cat.#: 112391

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Product Information

  • Product Name
    MAGED2 antibody
  • Documents
  • Description
    MAGED2 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, HepG2 cells, MCF7 cells. Positive IP detected in HepG2 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 65 kDa
  • Tested applications
    ELISA, WB, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    11B6 antibody; HCA10 antibody; JCL 1 antibody; MAGE D2 antibody; MAGE D2 antigen antibody; MAGED antibody; MAGED2 antibody; melanoma antigen family D antibody; Melanoma assOCiated antigen D2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MAGED2 recombinant protein (Accession Number: NM_201222). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:500-1:5000

    IF: 1:10-1:100

  • Validations

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:112391(MAGED2 antibody) at dilution of 1:1000

    A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:112391(MAGED2 antibody) at dilution of 1:1000

    Immunofluorescent analysis of Hela cells, using MAGED2 antibody Catalog No:112391 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using MAGED2 antibody Catalog No:112391 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-MAGED2 (IP:Catalog No:112391, 4ug; Detection:Catalog No:112391 1:1000) with HepG2 cells lysate 3200ug.

    IP Result of anti-MAGED2 (IP:Catalog No:112391, 4ug; Detection:Catalog No:112391 1:1000) with HepG2 cells lysate 3200ug.

  • Background
    MAGED2 represents melanoma antigen family D, 2
  • References
    • Pirlot C, Thiry M, Trussart C, Di Valentin E, Piette J, Habraken Y. Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress. Biochimica et biophysica acta. 1863(4):581-95. 2016.
    • Mouri A, Sasaki A, Watanabe K. MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(13):4562-80. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"