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Product Name
MAGED1 antibody
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Description
MAGED1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, Apoptosised HeLa cells, HeLa cells, human brain tissue. Positive FC detected in HeLa cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 66 kDa,92-95 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DLXIN 1 antibody; MAGE D1 antigen antibody; MAGE tumor antigen CCF antibody; MAGED1 antibody; melanoma antigen family D antibody; Melanoma assOCiated antigen D1 antibody; NRAGE antibody; PP2250 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MAGED1 recombinant protein (Accession Number: NM_001005333). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112389(MAGED1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:112389(MAGED1 Antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:112389(MAGED1 Antibody) at dilution of 1:100 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug MAGED1 antibody (Catalog No:112389, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"