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Product Name
MAGEA1 Polyclonal Antibody
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Description
Polyclonal antibody to MAGEA1
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
MAGEA1 antibody; CT1.1 antibody; MAGE1 antibody; MAGE family member A1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGEA1 (NP_004979.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - MAGEA1 Polyclonal Antibody
Western blot analysis of extracts of 22Rv1 cells, using MAGEA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - MAGEA1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human breast cancer using MAGEA1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - MAGEA1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver cancer using MAGEA1 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - MAGEA1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach cancer using MAGEA1 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - MAGEA1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using MAGEA1 antibody . Blue: DAPI for nuclear staining.
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Background
May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"