-
Product Name
MAFG Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to MAFG
-
Tested applications
WB
-
Species reactivity
Human
-
Alternative names
MAFG antibody; hMAF antibody; transcription factor MafG antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human MAFG (NP_002350.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - MAFG Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MAFG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
-
Background
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NFE2L2. Activates globin gene expression when associated with NFE2L2. May be involved in signal transduction of extracellular H(+) (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"