-
Product Name
MADCAM1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to MADCAM1
-
Tested applications
WB
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
MADCAM1 antibody; MACAM1 antibody; mucosal addressin cell adhesion molecule 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide of human MADCAM1
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:200 - 1:1000
-
Validations
Western blot - MADCAM1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MADCAM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
-
Background
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"