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Product Name
LZTR1 Polyclonal Antibody
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Description
Polyclonal antibody to LZTR1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
LZTR1 antibody; BTBD29 antibody; LZTR-1 antibody; NS10 antibody; SWNTS2 antibody; leucine zipper like transcription regulator 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-545 of human LZTR1 (NP_006758.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - LZTR1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LZTR1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - LZTR1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using LZTR1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - LZTR1 Polyclonal Antibody
Immunofluorescence analysis of Raw264.7 cells using LZTR1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates ubiquitination of Ras (K-Ras/KRAS, N-Ras/NRAS and H-Ras/HRAS), leading to inhibit Ras signaling by decreasing Ras association with membranes. Probably mediates non-degradative ubiquitination of Ras.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"