Human LSD1 (His & GST Tag) recombinant protein

Human LSD1 (His & GST Tag) recombinant protein

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Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.

Lysine-specific histone demethylase 1A

MKIAA0601; FAD-BINDING PROTEIN BRAF35-HDAC COMPLEX; BRAF35-HDAC COMPLEX PROTEIN BHC110; LYSINE (K)-SPECIFIC DEMETHYLASE 1; AMINE OXIDASE (FLAVIN CONTAINING) DOMAIN 2; BHC110; 1810043O07RIK; D4ERTD478E; LSD1; KDM1; RP1-184J9.1; RP23-426N4.2; 110 KDA SUBUNIT; LYSINE-SPECIFIC HISTONE DEMETHYLASE 1; LYSINE-SPECIFIC HISTONE DEMETHYLASE 1A; AOF2; FLAVIN-CONTAINING AMINE OXIDASE DOMAIN-CONTAINING PROTEIN 2; AA408884

O60341

KDM1A; AOF2; KDM1; KIAA0601; LSD1

Baculovirus-Insect Cells

A DNA sequence encoding the human KDM1 (O60341-1) (Ser172-Met852) was fused with the N-terminal poly histidine-tagged GST tag at the N-terminus.

Human

The recombinant human KDM1 /GST chimera consists of 917 amino acids and has a calculated molecular mass of 103.3 kDa. The recombinant protein migrates as an approximately 93 kDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE