-
Product Name
LSM2 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to LSM2
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human
-
Alternative names
LSM2 antibody; C6orf28 antibody; G7B antibody; YBL026W antibody; snRNP antibody; U6 snRNA-associated Sm-like protein LSm2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human LSM2 (NP_067000.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - LSM2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LSM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - LSM2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using LSM2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - LSM2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using LSM2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
-
Background
Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"