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Product Name
LRSAM1 antibody
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Description
LRSAM1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HepG2 cells, mouse liver tissue. Positive IHC detected in human skeletal muscle tissue. Positive IF detected in A431 cells. Observed molecular weight by Western-blot: 84 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
FLJ31641 antibody; hTAL antibody; LRSAM1 antibody; RIFLE antibody; TAL antibody; Tsg101 assOCiated ligase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LRSAM1 recombinant protein (Accession Number: NM_138361). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:112341(LRSAM1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:112341(LRSAM1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:112341(LRSAM1 Antibody) at dilution of 1:50
Immunofluorescent analysis of A431 cells using Catalog No:112341(LRSAM1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
LRSAM1 also named as hTAL, RIFLE orTsg101 associated ligase is a 723 amino acid protein, which contains 6 LRR repeats, 1 Ring-type zinc finger and 1 SAM domain. LRSAM1 is localizes in cytoplasm and highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. As a E3 ubiquitin protein ligase, LRSAM1 mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic and exocytic cargos, LRSAM1 gene mutation has an association with Charcot Maire Tooth disease 2P.
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