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Product Name
LPHN3 antibody
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Description
LPHN3 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive WB detected in mouse brain tissue, COLO 320 cells, HepG2 cells, human liver tissue, mouse colon tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 139 kDa
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Tested applications
ELISA, WB, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CIRL 3 antibody; CIRL3 antibody; KIAA0768 antibody; latrophilin 3 antibody; LEC3 antibody; Lectomedin 3 antibody; LPHN3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_015236). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112303(LPHN3 antibody) at dilution of 1:300
IP Result of anti-LPHN3 (IP:Catalog No:112303, 4ug; Detection:Catalog No:112303 1:300) with mouse brain tissue lysate 4000ug.
1X10^6 HepG2 cells were stained with .2ug LPHN3 antibody (Catalog No:112303, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.
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