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Product Name
LMAN1 antibody
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Description
LMAN1 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, HepG2 cells, MCF7 cells, mouse brain tissue. Positive IP detected in HepG2 cells. Positive IF detected in A549 cells. Positive IHC detected in human stomach cancer tissue. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ERGIC 53 antibody; ERGIC53 antibody; F5F8D antibody; FMFD1 antibody; gp58 antibody; Lectin mannose binding 1 antibody; lectin antibody; mannose binding antibody; LMAN1 antibody; MCFD1 antibody; MR60 antibody; Protein ERGIC 53 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LMAN1 recombinant protein (Accession Number: NM_005570). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112250(LMAN1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:112250(LMAN1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:112250(LMAN1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-LMAN1 (IP:Catalog No:112250, 4ug; Detection:Catalog No:112250 1:400) with HepG2 cells lysate 1000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed A549 cells using Catalog No:112250(LMAN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
LMAN1 represents lectin, mannose-binding, 1
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