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Product Name
LIPH antibody
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Description
LIPH Rabbit Polyclonal antibody. Positive WB detected in A549 cells, COLO 320 cells, mouse lung tissue. Positive IP detected in mouse lung tissue. Positive IHC detected in human pancreas tissue. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Lipase member H antibody; lipase antibody; member H antibody; LIPH antibody; LPD lipase related protein antibody; LPDLR antibody; mPA PLA1 antibody; mPA PLA1 alpha antibody; MPAPLA1 antibody; Phospholipase A1 member B antibody; PLA1B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LIPH recombinant protein (Accession Number: NM_139248). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:112243(LIPH antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:112243(LIPH antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:112243(LIPH antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-LIPH (IP:Catalog No:112243, 4ug; Detection:Catalog No:112243 1:500) with mouse lung tissue lysate 4000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed A549 cells using Catalog No:112243(LIPH Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).
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References
- Seki Y, Yoshida Y, Ishimine H. Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas. Biochemical and biophysical research communications. 443(4):1141-7. 2014.
- Cui M, Jin H, Shi X. Lipase member H is a novel secreted protein associated with a poor prognosis for breast cancer patients. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 35(11):11461-5. 2014.
- Ishimine H, Zhou R, Sumitomo K. Lipase member H frequently overexpressed in human esophageal adenocarcinomas. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"