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Product Name
LIPG Polyclonal Antibody
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Description
Polyclonal antibody to LIPG
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
LIPG antibody; EDL antibody; EL antibody; PRO719 antibody; endothelial lipase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 251-500 of human LIPG (NP_006024.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - LIPG Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LIPG antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - LIPG Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using LIPG antibody at dilution of 1:200 (40x lens).
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Background
Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"