LIP2 (human) peptide

LIP2 (human) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

LRSPRVLFSTQPPREAADTQDLDFEVGGAAPFNRTHRS

H-Leu-Arg-Ser-Pro-Arg-Val-Leu-Phe-Ser-Thr-Gln-Pro-Pro-Arg-Glu-Ala-Ala-Asp-Thr-Gln-Asp-Leu-Asp-Phe-Glu-Val-Gly-Gly-Ala-Ala-Pro-Phe-Asn-Arg-Thr-His-Arg-Ser-OH

38

95.8%

C₁₈₄H₂₈₈N₅₈O₅₈

4240.67

Synthetic

Powder

LIP2 is a 38 amino acid peptide derived from the nerve growth factor (NGF) proregion. The compound exerts multiple biological actvities it is neuroprotective in vitro and in vivo, inhibits the mitogenic activity of estrogen and IGF in MCF-7 breast cancer cells, stimulates phosphorylation of TrkA and Akt, and induces F-actin distribution. High levels of LIP2 were detected in the sera and the synovial fluid of rheumatoid arthritis patients.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• E.Dicou et al., J. Cell Biol., 136, 389 (1997)
• E.Dicou, Biochem. Biophys. Res. Commun., 347, 833 (2006)
• E.Dicou, J. Neuroimmunol., 194, 143 (2008)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.